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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Cholecystokinin ELISA kit (E07C0376)

E07C0376 Porcine Cholecystokinin ELISA kit

The Porcine Cholecystokinin ELISA kit is suitable for detecting samples from porcine species. Cholecystokinin can also be called CCK, CCK-PZ.


Products

Specifications of Porcine Cholecystokinin ELISA kit

Product Information

Cat. No.

E07C0376

Product Name

Porcine Cholecystokinin ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CCK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CCK antibody and an CCK-HRP conjugate. The assay sample and buffer are incubated together with CCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCK concentration since CCK from samples and CCK-HRP conjugate compete for the anti-CCK antibody binding site. Since the number of sites is limited, as more sites are occupied by CCK from the sample, fewer sites are left to bind CCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Cholecystokinin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

96-113%

Linearity

Diluent Ratio

Range %

1:2

97-110

1:4

95-108

1:8

96-104

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CCK and analogues was observed.


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Summary of the Assay Procedure for Porcine Cholecystokinin ELISA kit

Summary of the Assay Procedure for Porcine Cholecystokinin ELISA kit

Citations of Porcine Cholecystokinin ELISA kit

E07C0376 has been referenced in the below publications:

The heat treatment and the gelation are strong determinants of the kinetics of milk proteins digestion and of the peripheral availability of amino acids.

Acid and rennet gels exhibit strong differences in the kinetics of milk protein digestion and amino acid bioavailability.

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