En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Diamine Oxidase ELISA kit (E07D0032)

E07D0032 Porcine Diamine Oxidase ELISA kit

The Porcine Diamine Oxidase ELISA kit is suitable for detecting samples from porcine species. Diamine Oxidase can also be called Amiloride Binding Protein 1, ABP1, AOC1, KAO, Histaminase, Kidney amine oxidase, Amiloride-sensitive amine oxidase, Amine oxidase copper domain-containing protein 1.

Products

Specifications of Porcine Diamine Oxidase ELISA kit

Product Information

Cat. No.

E07D0032

Product Name

Porcine Diamine Oxidase ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFIDAOION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DAO ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DAO antibody and an DAO-HRP conjugate. The assay sample and buffer are incubated together with DAO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAO concentration since DAO from samples and DAO-HRP conjugate compete for the anti-DAO antibody binding site. Since the number of sites is limited, as more sites are occupied by DAO from the sample, fewer sites are left to bind DAO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAO concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Diamine Oxidase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-104%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

91-107

1:8

93-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between DAO and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Diamine Oxidase ELISA kit

Summary of the Assay Procedure for Porcine Diamine Oxidase ELISA kit

Citations of Porcine Diamine Oxidase ELISA kit

E07D0032 has been referenced in the below publications:

Low-protein diets supplemented with glutamic acid or aspartic acid ameliorates intestinal damage in weaned piglets challenged with hydrogen peroxide.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.