E07E0314 Porcine Estrogens ELISA kit
The Porcine Estrogens ELISA kit is suitable for the detection of samples from porcine species. Estrogens can also be called ES, oestrogen.
Specifications of Porcine Estrogens ELISA kit
Product Information | |
Cat. No. | E07E0314 |
Product Name | Porcine Estrogens ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ES ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ES antibody and an ES-HRP conjugate. The assay sample and buffer are incubated together with ES-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ES concentration since ES from samples and ES-HRP conjugate compete for the anti-ES antibody binding site. Since the number of sites is limited, as more sites are occupied by ES from the sample, fewer sites are left to bind ES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ES concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Estrogens ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-110% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-110 | |
1:4 | 89-107 | |
1:8 | 91-105 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ES and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Porcine Estrogens ELISA kit
Citations of Porcine Estrogens ELISA kit
E07E0314 has been referenced in the below publications:
Expression of CART Protein in Pig Ovarian Follicles and Effects on Folliclar Granulosa Cells in Vitro Culture.
