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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Basic Fibroblast Growth Factor ELISA kit (E07F0003)

E07F0003 Porcine Basic Fibroblast Growth Factor ELISA kit

Porcine Basic Fibroblast growth factor ELISA kit is suitable for the detection of samples from porcine species. Basic Fibroblast growth factor can also be called B-FGF, BFGF, FGFB, HBGH-2, Basic Fibroblast Growth Factor, Heparin-binding growth factor 2.

Products

Specifications of Porcine Basic Fibroblast Growth Factor ELISA kit

Product Information

Cat. No.

E07F0003

Product Name

Porcine Basic Fibroblast growth factor ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

bFGF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-bFGF antibody and an bFGF-HRP conjugate. The assay sample and buffer are incubated together with bFGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the bFGF concentration since bFGF from samples and bFGF-HRP conjugate compete for the anti-bFGF antibody binding site. Since the number of sites is limited, as more sites are occupied by bFGF from the sample, fewer sites are left to bind bFGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The bFGF concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Basic Fibroblast Growth Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-108%

Linearity

Diluent Ratio

Range %

1:2

93-106

1:4

91-104

1:8

95-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between bFGF and analogues was observed.



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Summary of the Assay Procedure for Porcine Basic Fibroblast Growth Factor ELISA kit

Summary of the Assay Procedure for Porcine Basic Fibroblast Growth Factor ELISA kit

Citations of Porcine Basic Fibroblast Growth Factor ELISA kit

E07F0003 has been referenced in the below publications:

Novel Insights on the Corpus Luteum Function:Role of Vaspin on Porcine Luteal Cell Angiogenesis, Proliferation and Apoptosis by Activation of GRP78 Receptor and MAP3/1 Kinase Pathways.

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