E07F0003 Porcine Basic Fibroblast Growth Factor ELISA kit
Porcine Basic Fibroblast growth factor ELISA kit is suitable for the detection of samples from porcine species. Basic Fibroblast growth factor can also be called B-FGF, BFGF, FGFB, HBGH-2, Basic Fibroblast Growth Factor, Heparin-binding growth factor 2.
Product Information | |
Cat. No. | E07F0003 |
Product Name | Porcine Basic Fibroblast growth factor ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
bFGF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-bFGF antibody and an bFGF-HRP conjugate. The assay sample and buffer are incubated together with bFGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the bFGF concentration since bFGF from samples and bFGF-HRP conjugate compete for the anti-bFGF antibody binding site. Since the number of sites is limited, as more sites are occupied by bFGF from the sample, fewer sites are left to bind bFGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The bFGF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-108% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-106 | |
1:4 | 91-104 | |
1:8 | 95-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between bFGF and analogues was observed. | |
E07F0003 has been referenced in the below publications:
Novel Insights on the Corpus Luteum Function:Role of Vaspin on Porcine Luteal Cell Angiogenesis, Proliferation and Apoptosis by Activation of GRP78 Receptor and MAP3/1 Kinase Pathways.
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