E07G0013 Porcine Ghrelin ELISA kit
The Porcine Ghrelin ELISA kit is suitable for the detection of samples from porcine species. Ghrelin can also be called Appetite regulating hormone, Ghrelin 27, Ghrelin 28, Ghrelin/obestatin prepropeptide, Growth hormone releasing peptide, Growth hormone secretagogue, M46 protein, Motilin related peptide, MTLRP, Obestatin, Obestatin preprohormone.
Product Information | |
Cat. No. | E07G0013 |
Product Name | Porcine Ghrelin ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Ghrelin ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Ghrelin antibody and an Ghrelin-HRP conjugate. The assay sample and buffer are incubated together with Ghrelin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ghrelin concentration since Ghrelin from samples and Ghrelin-HRP conjugate compete for the anti-Ghrelin antibody binding site. Since the number of sites is limited, as more sites are occupied by Ghrelin from the sample, fewer sites are left to bind Ghrelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ghrelin concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 94-108% | |
Linearity | Diluent Ratio | Range % |
1:2 | 97-114 | |
1:4 | 93-105 | |
1:8 | 91-106 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Ghrelin and analogues was observed. | |
E07G0013 has been referenced in the below publications:
The heat treatment and the gelation are strong determinants of the kinetics of milk proteins digestion and of the peripheral availability of amino acids.
Acid and rennet gels exhibit strong differences in the kinetics of milk protein digestion and amino acid bioavailability.
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