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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine 26S Proteasome ELISA kit (E08A0669)

E08A0669 Canine 26S Proteasome ELISA kit

The canine 26S proteasome ELISA kit is suitable for the detection of samples from Canine species. 26S proteasome can also be called 26S PSM, 26SPSM, 26S protease complex, ATP-dependent 26S proteasome, 26S proteasome regulatory complex, 26S proteolytic complex, 26S proteasome complex, Multi-catalytic proteinase complex, Proteasome 26S non-ATPase regulatory subunit, 26S proteasome subunit, ATPase, Proteasome subunit alpha type 6, PRC2 (proteasome regulatory particle complex 2).

Products

Specifications of Canine 26S Proteasome ELISA kit

Product Information

Cat. No.

E08A0669

Product Name

Canine 26S proteasome ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

26S PSM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-26S PSM antibody and an 26S PSM-HRP conjugate. The assay sample and buffer are incubated together with 26S PSM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 26S PSM concentration since 26S PSM from samples and 26S PSM-HRP conjugate compete for the anti-26S PSM antibody binding site. Since the number of sites is limited, as more sites are occupied by 26S PSM from the sample, fewer sites are left to bind 26S PSM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 26S PSM concentration in each sample is interpolated from this standard curve.


Quality Control on Canine 26S Proteasome ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

88-106%

Linearity

Diluent Ratio

Range %

1:2

86-105

1:4

83-108

1:8

84-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 26S PSM and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Canine 26S Proteasome ELISA kit

Summary of the Assay Procedure for Canine 26S Proteasome ELISA kit

Citations of Canine 26S Proteasome ELISA kit

E08A0669 has been referenced in the below publications:

26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumors.

Evaluation of serum insulin-like growth factor-1 and 26S proteasome concentrations in healthy dogs and dogs with chronic diseases depending on body condition score.


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