Name: Canine 26S Proteasome ELISA kit (E08A0669)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Canine 26S Proteasome ELISA kit (E08A0669)

E08A0669 Canine 26S Proteasome ELISA kit

The canine 26S proteasome ELISA kit is suitable for the detection of samples from Canine species. 26S proteasome can also be called 26S PSM, 26SPSM, 26S protease complex, ATP-dependent 26S proteasome, 26S proteasome regulatory complex, 26S proteolytic complex, 26S proteasome complex, Multi-catalytic proteinase complex, Proteasome 26S non-ATPase regulatory subunit, 26S proteasome subunit, ATPase, Proteasome subunit alpha type 6, PRC2 (proteasome regulatory particle complex 2).

Products

Specifications of Canine 26S Proteasome ELISA kit

Product Information
Cat. No.	E08A0669
Product Name	Canine 26S proteasome ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

26S PSM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-26S PSM antibody and an 26S PSM-HRP conjugate. The assay sample and buffer are incubated together with 26S PSM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 26S PSM concentration since 26S PSM from samples and 26S PSM-HRP conjugate compete for the anti-26S PSM antibody binding site. Since the number of sites is limited, as more sites are occupied by 26S PSM from the sample, fewer sites are left to bind 26S PSM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 26S PSM concentration in each sample is interpolated from this standard curve.

Quality Control on Canine 26S Proteasome ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	88-106%
Linearity	Diluent Ratio	Range %
	1:2	86-105
	1:4	83-108
	1:8	84-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between 26S PSM and analogues was observed.

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Summary of the Assay Procedure for Canine 26S Proteasome ELISA kit

Citations of Canine 26S Proteasome ELISA kit

E08A0669 has been referenced in the below publications:

26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumors.

Evaluation of serum insulin-like growth factor-1 and 26S proteasome concentrations in healthy dogs and dogs with chronic diseases depending on body condition score.