E08A0851 Canine Apoprotein B100 ELISA kit
Canine Apoprotein B100 ELISA kit is suitable for the detection of samples from Canine species. Apoprotein B100 can also be called APOB, FLDB, LDLCQ4, apoB-100, apoB-48, and apoB100.
Specifications of Canine Apoprotein B100 ELISA kit
Product Information | |
Cat. No. | E08A0851 |
Product Name | Canine Apoprotein B100 ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 μg/mL |
Sensitivity | 0.1 μg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 μg/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 μg/mL | 1 vial |
STANDARD C (0.5mL) | 1 μg/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 μg/mL | 1 vial |
STANDARD E (0.5mL) | 5 μg/mL | 1 vial |
STANDARD F (0.5mL) | 10 μg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Apo B100 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Apo B100 antibody and an Apo B100-HRP conjugate. The assay sample and buffer are incubated together with Apo B100-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Apo B100 concentration since Apo B100 from samples and Apo B100-HRP conjugate compete for the anti-Apo B100 antibody binding site. Since the number of sites is limited, as more sites are occupied by Apo B100 from the sample, fewer sites are left to bind Apo B100-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo B100 concentration in each sample is interpolated from this standard curve. |
Quality Control on Canine Apoprotein B100 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 85-109% | |
Linearity | Diluent Ratio | Range % |
1:2 | 85-108 | |
1:4 | 83-111 | |
1:8 | 84-114 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Apo B100 and analogues was observed. | |
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Summary of the Assay Procedure for Canine Apoprotein B100 ELISA kit
Citations of Canine Apoprotein B100 ELISA kit
E08A0851 has been referenced in the below publications:
Serum proteome profiling in canine chronic valve disease using a TMT-based quantitative proteomics approach.
