E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit
Canine Brain Derived Neurotrophic Factor ELISA kit is suitable for the detection of samples from Canine species. Brain Derived Neurotrophic Factor can also be called Neurotrophin, Abrineurin, BDNF.
Product Information | |
Cat. No. | E08B0029 |
Product Name | Canine Brain Derived Neurotrophic Factor ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BDNF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BDNF. Standards or samples are then added to the microtiter plate wells and BDNF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BDNF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BDNF are added to each well to “sandwich” the BDNF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BDNF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDNF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-103% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-105 | |
1:4 | 86-105 | |
1:8 | 83-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BDNF and analogues was observed. | |
E08B0029 has been referenced in the below publications:
Effects of Electroacupuncture on Plasma Concentrations of Three Related Neurotrophic Factors in Normal Dogs.
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