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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Galectin 3 ELISA kit (E08G0052)

E08G0052 Canine Galectin 3 ELISA Kit

Canine Galectin 3 ELISA kit is suitable for the detection of samples from Canine species. Galectin 3 can also be called LGALS3, CBP35, GALBP, GALIG, MAC2, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, Laminin-binding protein, Lectin L-29, GAL3.

Products

Specifications of Canine Galectin 3 ELISA kit

Product Information

Cat. No.

E08G0052

Product Name

Canine Galectin 3 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GAL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GAL3 antibody and an GAL3-HRP conjugate. The assay sample and buffer are incubated together with GAL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAL3 concentration since GAL3 from samples and GAL3-HRP conjugate compete for the anti-GAL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAL3 from the sample, fewer sites are left to bind GAL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL3 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Galectin 3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

87-108

1:4

86-111

1:8

83-113

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GAL3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Coefficient of Variance

Summary of the Assay Procedure for Coefficient of Variance

Citations of Canine Galectin 3 ELISA kit

E08G0052 has been referenced in the below publications:

Changes of cardiac function in diabetic dogs.

Galectin-3 in cardiac muscle and circulation of dogs with degenerative mitral valve disease.

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