E11A0043 Bovine Adenosine Monophosphate Activated Protein ELISA kit
Bovine Adenosine Monophosphate Activated Protein ELISA kit is suitable for the detection of samples from Bovine species. Adenosine Monophosphate Activated Protein can also be called PRKAa1, AMPKa1, PKA, ACACA kinase, AMPK.
Product Information | |
Cat. No. | E11A0043 |
Product Name | Bovine Adenosine Monophosphate Activated Protein ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AMPK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMPK antibody and an AMPK-HRP conjugate. The assay sample and buffer are incubated together with AMPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMPK concentration since AMPK from samples and AMPK-HRP conjugate compete for the anti-AMPK antibody binding site. Since the number of sites is limited, as more sites are occupied by AMPK from the sample, fewer sites are left to bind AMPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPK concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-101% | |
Linearity | Diluent Ratio | Range % |
1:2 | 85-103 | |
1:4 | 84-108 | |
1:8 | 82-107 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AMPK and analogues was observed. | |
E11A0043 has been referenced in the below publications：
β-Hydroxybutyric Sodium Salt Inhibition of Growth Hormone and Prolactin Secretion via the cAMP/PKA/CREB and AMPK Signaling Pathways in Dairy Cow Anterior Pituitary Cells.
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