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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Carnitine Palmitoyltransferase I ELISA kit (E11C0055)

E11C0055 Bovine Carnitine Palmitoyltransferase I ELISA kit

Bovine Carnitine palmitoyltransferase I ELISA kit is suitable for the detection of samples from Bovine species. Carnitine palmitoyltransferase I can also be called arnitine acyltransferase I, CPTI, CAT1, CoA, carnitine acyl transferase (CCAT), or palmitoyl CoA transferase I, CPT 1, CPT-1.

Products

Specifications of Bovine Carnitine Palmitoyltransferase I ELISA kit

Product Information

Cat. No.

E11C0055

Product Name

Bovine Carnitine palmitoyltransferase I ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

The CPT 1 ELISA kit uses an anti-CPT 1 antibody and a CPT 1-HRP conjugate to perform a competitive enzyme immunoassay. The CPT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT 1 concentration since CPT 1 from samples and CPT 1-HRP conjugate compete for the anti-CPT 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT 1 from the sample, fewer sites are left to bind CPT 1-HRP conjugate. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. From this standard curve, the CPT 1 concentration in each sample is extrapolated.


Quality Control on Bovine Carnitine Palmitoyltransferase I ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CPT 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Carnitine Palmitoyltransferase I ELISA kit

Summary of the Assay Procedure for Bovine Carnitine Palmitoyltransferase I ELISA kit

Citations of Bovine Carnitine Palmitoyltransferase I ELISA kit

E11C0055 has been referenced in the below publications:

Non-Esterified Fatty Acids Activate the AMP-Activated Protein Kinase Signaling Pathway to Regulate Lipid Metabolism in Bovine Hepatocytes.

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