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BlueGene Biotech Chicken Total Adiponectin ELISA kit (E12A0125)

  • Signal Transduction
  • Cardiovascular
  • Neuroscience
  • Stem Cells
  • Metabolism

E12A0125 Chicken Total Adiponectin ELISA kit

Chicken Total Adiponectin ELISA kit (a knid of chicken elisa kits )is suitable for the detection of samples from chicken species. Total Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, C1q and collagen domain-containing protein, GBP 28, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein.

Products

Specifications of Chicken Total Adiponectin ELISA kit

Product Information

Cat. No.

E12A0125

Product Name

Chicken Total Adiponectin ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPN concentration since ADPN from samples and ADPN-HRP conjugate compete for the anti-ADPN antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPN from the sample, fewer sites are left to bind ADPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Total Adiponectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-116.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

92-105

1:8

88 -103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ADPN  and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Total Adiponectin ELISA kit

Summary of the Assay Procedure for Chicken Total Adiponectin ELISA kit

Citations of Chicken Total Adiponectin ELISA kit

E12A0125 has been referenced in the below publications:

Quercetin: Effects on Endocrine Factor Levels in Adipocytes of Broilers.

Expression profile of adiponectin and adiponectin receptors in high-fat diet feeding chickens.

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