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BlueGene Biotech Chicken β Cell Leukemia/Lymphoma 2 ELISA kit (E12B0033)

  • Cell Biology
  • Signal Transduction
  • Cancer
  • Metabolism

E12B0033 Chicken β Cell Leukemia/Lymphoma 2 ELISA kit

Chicken β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samples from chicken species. β Cell Leukemia/Lymphoma 2 can also be called BCL2, Bcl-2, PPP1R50, B-cell CLL/lymphoma 2, apoptosis regulator, BCL2 apoptosis regulator, Genes, bcl-2.

Products

Specifications Of E12B0033 Chicken BCL 2 ELISA Kit

Product Information

Cat. No.

E12B0033

Product Name

Chicken β Cell Leukemia/Lymphoma 2 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BCL 2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- BCL 2 antibody and a BCL 2-HRP conjugate. The assay sample and buffer are incubated together with BCL 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL 2 concentration since BCL 2 from samples and   BCL 2-HRP conjugate compete for the anti-BCL 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL 2 from the sample, fewer sites are left to bind BCL 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL 2 concentration in each sample is interpolated from this standard curve.


Quality Control On Chicken BCL 2 ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

86-117.48

Linearity

Diluent ratio

Range %

1:2

95-116

1:4

92-103

1:8

85 -101

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between BCL 2 and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E12B0033 Chicken BCL 2 ELISA Kit

Summary Of The Assay Procedures For E12B0033 Chicken BCL 2 ELISA Kit

Citations Of Chicken β Cell Leukemia/Lymphoma 2 ELISA Kit

Experimental study of the inhibition effect of ''Phrul sByor Chem Mo'': a Tibetan medical prescription, on digestive system tumour cell lines in vitro 

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