E12B0764 Chicken Bcl 2 like protein 1 ELISA kit
Chicken Bcl 2 like protein 1 ELISA kit is suitable for the detection of samples from chicken species. Bcl 2 like protein 1 can also be called BCL2L1, Bcl2l1,Bcl(X)L, Bcl-XL, Bcl2l, BclX, bcl-x, bcl2-L-1, BCL-XL/S, BCLXL, BCLXS, PPP1R52, bcl-xS, BCL2L, BCLX, Bcl-X, bcl-xL, BCL2 like 1.
Product Information | |
Cat. No. | E12B0764 |
Product Name | Chicken Bcl 2 like protein 1 (BCL2L1)ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BCL2L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BCL2L1 antibody and a BCL2L1-HRP conjugate. The assay sample and buffer are incubated together with BCL2L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2L1 concentration since BCL2L1 from samples and BCL2L1-HRP conjugate compete for the anti-BCL2L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2L1 from the sample, fewer sites are left to bind BCL2L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCL2L1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-115.87% | |
Linearity | Diluent Ratio | Range % |
1:2 | 97-117 | |
1:4 | 94-104 | |
1:8 | 86 -101 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BCL2L1 and analogues was observed. | |
E12B0764 has been referenced in the below publications:
Roles of miRNA and MDV oncogene Meq in the transformation of chicken Marek's disease lymphoma.
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