E17C0027 Fish Cyclic Adenosine Monophosphate ELISA kit
Fish cyclic Adenosine Monophosphate ELISA kit is suitable for the detection of samples from fish species. Cyclic Adenosine Monophosphate can also be called cAMP, AMP or 3',5'-cyclic adenosine monophosphate.
Product Information | |
Cat. No. | E17C0027 |
Product Name | Fish cyclic Adenosine Monophosphate ELISA kit |
Species | Fish |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pmol/ml |
Sensitivity | 1.0 pmol/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pmol/ml | 1 vial |
STANDARD B (0.5mL) | 50 pmol/ml | 1 vial |
STANDARD C (0.5mL) | 100 pmol/ml | 1 vial |
STANDARD D (0.5mL) | 250 pmol/ml | 1 vial |
STANDARD E (0.5mL) | 500 pmol/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pmol/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
cAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cAMP antibody and an cAMP-HRP conjugate. The assay sample and buffer are incubated together with cAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cAMP concentration since cAMP from samples and cAMP-HRP conjugate compete for the anti-cAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 85-113.86% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-112 | |
1:4 | 91-108 | |
1:8 | 83-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between cAMP and analogues was observed. | |
E17C0027 Fish Cyclic Adenosine Monophosphate ELISA kit
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