E17P0666 Fish Pyruvate Kinase A ELISA kit
Fish Pyruvate Kinase A ELISA kit can be used to identify samples from the fish species. Pyruvate Kinase A can also be called cAMP-dependent protein kinase, PKA.
Specifications of Fish Pyruvate Kinase A ELISA kit
Product Information | |
Cat. No. | E17P0666 |
Product Name | Fish Pyruvate Kinase A ELISA kit |
Species | Fish |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PKA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PKA antibody and an PKA-HRP conjugate. The assay sample and buffer are incubated together with PKA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKA concentration since PKA from samples and PKA-HRP conjugate compete for the anti-PKA antibody binding site. Since the number of sites is limited, as more sites are occupied by PKA from the sample, fewer sites are left to bind PKA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKA concentration in each sample is interpolated from this standard curve. |
Quality Control on Fish Pyruvate Kinase A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PKA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Fish Pyruvate Kinase A ELISA kit
Citations of Fish Pyruvate Kinase A ELISA kit
E17P0666 has been referenced in the below publications：
Developmental toxicity by thifluzamide in zebrafish (Danio rerio): Involvement of leptin.
