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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Fish Pyruvate Kinase A ELISA kit (E17P0666)

E17P0666 Fish Pyruvate Kinase A ELISA kit

Fish Pyruvate Kinase A ELISA kit can be used to identify samples from the fish species. Pyruvate Kinase A can also be called cAMP-dependent protein kinase, PKA.

Products

Specifications of Fish Pyruvate Kinase A ELISA kit

Product Information

Cat. No.

E17P0666

Product Name

Fish Pyruvate Kinase A ELISA kit

Species

Fish

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PKA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PKA antibody and an PKA-HRP conjugate. The assay sample and buffer are incubated together with PKA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKA concentration since PKA from samples and PKA-HRP conjugate compete for the anti-PKA antibody binding site. Since the number of sites is limited, as more sites are occupied by PKA from the sample, fewer sites are left to bind PKA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKA concentration in each sample is interpolated from this standard curve.


Quality Control on Fish Pyruvate Kinase A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PKA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Fish Pyruvate Kinase A ELISA kit

Summary of the Assay Procedure for Fish Pyruvate Kinase A ELISA kit

Citations of Fish Pyruvate Kinase A ELISA kit

E17P0666 has been referenced in the below publications:

Developmental toxicity by thifluzamide in zebrafish (Danio rerio): Involvement of leptin.



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