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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Heat Shock Protein 70 ELISA kit (E06H0022)

E06H0022 Goat Heat Shock Protein 70 ELISA kit

Goat Heat Shock Protein 70 ELISA kit is suitable for the detection of samples from goat species. Heat Shock Protein 70 can also be called Hsp70, DnaK, 70 Kilodalton Heat Shock Proteins.

Products

Specifications of Goat Heat Shock Protein 70 ELISA kit

Product Information

Cat. No.

E06H0022

Product Name

Goat Heat Shock Protein 70 ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HSP70 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HSP70 antibody and an HSP70-HRP conjugate. The assay sample and buffer are incubated together with HSP70-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP70 concentration since HSP70 from samples and HSP70-HRP conjugate compete for the anti- HSP70 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP70 from the sample, fewer sites are left to bind HSP70 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP70 concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Heat Shock Protein 70 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-112.65%

Linearity

Diluent Ratio

Range %

1:2

97-116

1:4

92-104

1:8

89-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HSP70 and analogues was observed.


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Summary of the Assay Procedure for Goat Heat Shock Protein 70 ELISA kit

Summary of the Assay Procedure for Goat Heat Shock Protein 70 ELISA kit

Citations of Goat Heat Shock Protein 70 ELISA kit

E06H0022 has been referenced in the below publications:

Adaptive capability as indicated by behavioral and physiological responses, plasma HSP70 level, and PBMC HSP70 mRNA expression in Osmanabadi goats subjected to combined (heat and nutritional) stressors.

Dynamics of HSPA1A and redox status in the spermatozoa and fluid from different segments of goat epididymis.

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