E06H0039 Goat Heparin binding protein ELISA kit
Goat Heparin binding protein ELISA kit is suitable for the detection of samples from Goat species. Heparin binding protein can also be called AAZAMP, AZU1, HBP, HUMAZUR, NAZC, CAP37, Cationic Antimicrobial Protein 37, Heparin-Binding Protein, Neutrophil Azurocidin, AZU.
Product Information | |
Cat. NO. | E06H0039 |
Product Name | Goat Heparin binding protein ELISA kit |
Species | Goat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| A competitive enzyme immunoassay approach is used in the AZU/HBP ELISA kit along with an anti-AZU/HBP antibody and an AZU/HBP-HRP conjugate. For one hour, the AZU/HBP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the AZU/HBP concentration since AZU/HBP from samples and AZU/HBP-HRP conjugate compete for the anti-AZU/HBP antibody binding site. Since the number of sites is limited, as more sites are occupied by AZU/HBP from the sample, fewer sites are left to bind AZU/HBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AZU/HBP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 89-109 | |
1:4 | 88-112 | |
1:8 | 85-111 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AZU/HBP and analogues was observed. | |
E06H0039 has been referenced in the below publications：
Diagnostic accuracy of urine heparin binding protein for pediatric acute pyelonephritis.
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