En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Heparin binding protein ELISA kit (E06H0039)

  • Metabolism

E06H0039 Goat Heparin binding protein ELISA kit

Goat Heparin binding protein ELISA kit is suitable for the detection of samples from Goat species. Heparin binding protein can also be called AAZAMP, AZU1, HBP, HUMAZUR, NAZC, CAP37, Cationic Antimicrobial Protein 37, Heparin-Binding Protein, Neutrophil Azurocidin, AZU.

Products

Specifications of Goat Heparin binding protein ELISA kit

Product Information

Cat. NO.

E06H0039

Product Name

Goat Heparin binding protein ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

A competitive enzyme immunoassay approach is used in the AZU/HBP ELISA kit along with an anti-AZU/HBP antibody and an AZU/HBP-HRP conjugate. For one hour, the AZU/HBP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the AZU/HBP concentration since AZU/HBP from samples and AZU/HBP-HRP conjugate compete for the anti-AZU/HBP antibody binding site. Since the number of sites is limited, as more sites are occupied by AZU/HBP from the sample, fewer sites are left to bind AZU/HBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AZU/HBP concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Heparin binding protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AZU/HBP and analogues was observed.


Summary of the Assay Procedure for Goat Heparin binding protein ELISA kit

BlueGene Biotech Product Show

Citations of Goat Heparin binding protein ELISA kit

E06H0039 has been referenced in the below publications:

Diagnostic accuracy of urine heparin binding protein for pediatric acute pyelonephritis.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.