Name: Goat Hypoxia Inducible Factor 1, Alpha ELISA kit (E06H0061)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Goat Hypoxia Inducible Factor 1, Alpha ELISA kit (E06H0061)

E06H0061 Goat Hypoxia Inducible Factor 1, Alpha ELISA kit

Goat Hypoxia Inducible Factor 1, Alpha ELISA kit is suitable for the detection of samples from Goat species. Hypoxia Inducible Factor 1, Alpha can also be called HIF1A, HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, HIF-1α, HIF 1Alpha.

Products

Specifications of Goat Hypoxia Inducible Factor 1, Alpha ELISA kit

Product Information
Cat. NO.	E06H0061
Product Name	Goat Hypoxia Inducible Factor 1, Alpha ELISA kit
Species	Goat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

A competitive enzyme immunoassay approach is used in the HIF 1Alpha ELISA kit along with an anti-HIF 1Alpha antibody and an HIF 1Alpha-HRP conjugate. For one hour, the HIF 1Alpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the HIF 1Alpha concentration since HIF 1Alpha from samples and HIF 1Alpha-HRP conjugate compete for the anti-HIF 1Alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by HIF 1Alpha from the sample, fewer sites are left to bind HIF 1Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIF 1Alpha concentration in each sample is interpolated from this standard curve.

Quality Control on Goat Hypoxia Inducible Factor 1, Alpha ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	92-106%
Linearity	Diluent Ratio	Range %
	1:2	89-109
	1:4	88-112
	1:8	85-111
Specificity/Cross-reactivity	No significant cross-reactivity or interference between HIF 1Alpha and analogues was observed.