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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Insulin ELISA kit (E06I0004)

E06I0004 Goat Insulin ELISA kit

Goat Insulin ELISA kit is suitable for the detection of samples from Goat species. Insulin can also be called ILPR, Insulin A chain, Insulin B chain, IRDN, INS.

Products

Specifications of Goat Insulin ELISA kit

Product Information

Cat. NO.

E06I0004

Product Name

Goat Insulin ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

A competitive enzyme immunoassay approach is used in the INS ELISA kit along with an anti-INS antibody and an INS-HRP conjugate. For one hour, the INS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the INS concentration since INS from samples and INS-HRP conjugate compete for the anti-INS antibody binding site. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Insulin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between INS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Goat Insulin ELISA kit

Summary of the Assay Procedure for Goat Insulin ELISA kit

Citations of Goat Insulin ELISA kit

E06I0004 has been referenced in the below publications:

EFFECT OF THE DIFFERCE DIETS OF CONCENTRATE TO FORAGE RATIO ON THE AMINO ACIDS METABOLISM IN LIVER AND RELATIVE HORMONE RESPONSE OF NONLACTATING GOATS.

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