En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Progesterone ELISA kit (E06P0200)

E06P0200 Goat Progesterone ELISA kit

Goat Progesterone ELISA kit is suitable for the detection of samples from goat species. Progesterone can also be called P4, Pregn-4-Ene-3,20-Dione, PROG.

Products

Specifications of Goat Progesterone ELISA kit

Product Information

Cat. NO.

E06P0200

Product Name

Goat Progesterone ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

A competitive enzyme immunoassay approach is used in the PROG ELISA kit along with an anti-PROG antibody and an PROG-HRP conjugate. For one hour, the PROG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the PROG concentration since PROG from samples and PROG-HRP conjugate compete for the anti-PROG antibody binding site. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROG concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Progesterone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PROG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Goat Progesterone ELISA kit

Summary of the Assay Procedure for Goat Progesterone ELISA kit

Citations of Goat Progesterone ELISA kit

E06P0200 has been referenced in the below publications:

Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.