E06P0200 Goat Progesterone ELISA kit
Goat Progesterone ELISA kit is suitable for the detection of samples from goat species. Progesterone can also be called P4, Pregn-4-Ene-3,20-Dione, PROG.
Specifications of Goat Progesterone ELISA kit
Product Information | |
Cat. NO. | E06P0200 |
Product Name | Goat Progesterone ELISA kit |
Species | Goat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
A competitive enzyme immunoassay approach is used in the PROG ELISA kit along with an anti-PROG antibody and an PROG-HRP conjugate. For one hour, the PROG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the PROG concentration since PROG from samples and PROG-HRP conjugate compete for the anti-PROG antibody binding site. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROG concentration in each sample is interpolated from this standard curve. |
Quality Control on Goat Progesterone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 89-109 | |
1:4 | 88-112 | |
1:8 | 85-111 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PROG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Goat Progesterone ELISA kit
Citations of Goat Progesterone ELISA kit
E06P0200 has been referenced in the below publications：
Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region.
