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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Prostaglandin F Metabolite ELISA kit (E06P0751)

E06P0751 Goat Prostaglandin F Metabolite ELISA kit

Goat Prostaglandin F Metabolite ELISA kit is suitable for the detection of samples from Goat species. Prostaglandin F Metabolite can also be called tromethamine, PGF2 α, Prostaglandin F2 Alpha, PGF2 Alpha.

Products

Specifications of Goat Prostaglandin F Metabolite ELISA kit

Product Information

Cat. NO.

E06P0751

Product Name

Goat Prostaglandin F Metabolite ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

A competitive enzyme immunoassay approach is used in the PGFM ELISA kit along with an anti-PGFM antibody and an PGFM-HRP conjugate. For one hour, the PGFM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the PGFM concentration since PGFM from samples and PGFM-HRP conjugate compete for the anti-PGFM antibody binding site. Since the number of sites is limited, as more sites are occupied by PGFM from the sample, fewer sites are left to bind PGFM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGFM concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Prostaglandin F Metabolite ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PGFM and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Goat Prostaglandin F Metabolite ELISA kit

Summary of the Assay Procedure for Goat Prostaglandin F Metabolite ELISA kit

Citations of Goat Prostaglandin F Metabolite ELISA kit

E06P0751 has been referenced in the below publications:

Effect of n-3 PUFA-rich fish oil supplementation during late gestation on kidding, uterine involution and resumption of follicular activity in goat.


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