E06T0758 Goat Transient Receptor Potential Vanilloid-6 ELISA kit
Goat Transient Receptor Potential Vanilloid-6 ELISA kit is suitable for the detection of samples from Goat species. Transient Receptor Potential Vanilloid-6 can also be called CAT1, CATL, ABP/ZF, ECAC2, ZFAB, Epithelial Calcium Channel 2.
Product Information | |
Cat. NO. | E06T0758 |
Product Name | Goat Transient Receptor Potential Vanilloid-6 ELISA kit |
Species | Goat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
A competitive enzyme immunoassay approach is used in the TRPV6 ELISA kit along with an anti-TRPV6 antibody and a TRPV6-HRP conjugate. For one hour, the TRPV6-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the TRPV6 concentration since TRPV6 from samples and TRPV6-HRP conjugate compete for the anti-TRPV6 antibody binding site. Since the number of sites is limited, as more sites are occupied by TRPV6 from the sample, fewer sites are left to bind TRPV6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRPV6 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 89-109 | |
1:4 | 88-112 | |
1:8 | 85-111 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TRPV6 and analogues was observed. | |
E06T0758 has been referenced in the below publications：
Synergy effects of lowering dietary cation-anion difference with VD and Mg supplementation on hypocalcemia prevention.
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