Name: Goat Vitamin D Receptor ELISA kit (E06V0028)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Goat Vitamin D Receptor ELISA kit (E06V0028)

E06V0028 Goat Vitamin D Receptor ELISA kit

Goat Vitamin D Receptor ELISA kit is suitable for the detection of samples from goat species. Vitamin D Receptor can also be called VDR, NR1I1, PPP1R163, vitamin D (1,25- dihydroxyvitamin D3) receptor, vitamin D receptor, VD R.

Products

Specifications of Goat Vitamin D Receptor ELISA kit

Product Information
Cat. NO.	E06V0028
Product Name	Goat Vitamin D Receptor ELISA kit
Species	Goat
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

A competitive enzyme immunoassay approach is used in the VD R ELISA kit along with an anti-VD R antibody and an VD R-HRP conjugate. For one hour, the VD R-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the VD R concentration since VD R from samples and VD R-HRP conjugate compete for the anti-VD R antibody binding site. Since the number of sites is limited, as more sites are occupied by VD R from the sample, fewer sites are left to bind VD R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VD R concentration in each sample is interpolated from this standard curve.

Quality Control on Goat Vitamin D Receptor ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	92-106%
Linearity	Diluent Ratio	Range %
	1:2	89-109
	1:4	88-112
	1:8	85-111
Specificity/Cross-reactivity	No significant cross-reactivity or interference between VD R and analogues was observed.