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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Guinea Pig Follicle Stimulating Hormone ELISA kit (E05F0001)

E05F0001 Guinea Pig Follicle Stimulating Hormone ELISA kit

Guinea Pig Follicle Stimulating Hormone ELISA kit is suitable for the detection of samples from Guinea Pig species. Follicle Stimulating Hormone can also be called Follicle Stimulating Hormone, Follicle stimulating hormone beta polypeptide, Follicle stimulating hormone beta subunit, Follitropin beta chain, Follitropin beta chain precursor, Follitropin subunit beta, FSH B, FSH-B, FSH-beta, FSHB, FSHbeta , FSH.

Products

Specifications of Guinea Pig Follicle Stimulating Hormone ELISA kit

Product Information

Cat. NO.

E05F0001

Product Name

Guinea Pig Follicle Stimulating Hormone ELISA kit

Species

Guinea Pig

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FSH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FSH antibody and an FSH-HRP conjugate. The assay sample and buffer are incubated together with FSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FSH antibody binding site between FSH from samples and FSH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FSH. As more sites are taken up by FSH from the sample, fewer sites are left that can bind FSH-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the FSH concentration in each sample.


Quality Control on Guinea Pig Follicle Stimulating Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-106%

Linearity

Diluent Ratio

Range %

1:2

93-103

1:4

89-108

1:8

86-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FSH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Guinea Pig Follicle Stimulating Hormone ELISA kit

Summary of the Assay Procedure for Guinea Pig Follicle Stimulating Hormone ELISA kit

Citations of Guinea Pig Follicle Stimulating Hormone ELISA kit

E05F0001 has been referenced in the below publications:

Effect of thymectomy on the female reproductive cycle in neonatal guinea pigs.

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