E05L0037 Guinea Pig Leukotriene B4 ELISA kit
Guinea Pig Leukotriene B4 ELISA kit is suitable for the detection of samples from Guinea Pig species. Leukotriene B4 can also be called LTB4, LT-B4.
Specifications of Guinea Pig Leukotriene B4 ELISA kit
Product Information | |
Cat. NO. | E05L0037 |
Product Name | Guinea Pig Leukotriene B4 ELISA kit |
Species | Guinea Pig |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LTB4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LTB4 antibody and an LTB4-HRP conjugate. The assay sample and buffer are incubated together with LTB4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LTB4 antibody binding site between LTB4 from samples and LTB4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LTB4. As more sites are taken up by LTB4 from the sample, fewer sites are left that can bind LTB4-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the LTB4 concentration in each sample. |
Quality Control on Guinea Pig Leukotriene B4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-103 | |
1:4 | 89-108 | |
1:8 | 86-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LTB4 and analogues was observed. | |
BlueGene Biotech Product
Summary of the Assay Procedure for Guinea Pig Leukotriene B4 ELISA kit
Citations of Guinea Pig Leukotriene B4 ELISA kit
E05L0037 has been referenced in the below publications：
Effects of Eliminating Wind and Dispersing Lung Formula on HIS, LTB4 and NE in plasma of the guinea pigs with airway hypersensitivity.
