E05T0007 Guinea Pig Testosterone ELISA kit
The Guinea Pig Testosterone ELISA kit is suitable for the detection of samples from Guinea Pig species. Testosterone can also be called TESTO, androgen, anabolic steroid.
Specifications of Guinea Pig Testosterone ELISA kit
Product Information | |
Cat. NO. | E05T0007 |
Product Name | Guinea Pig Testosterone ELISA kit |
Species | Guinea Pig |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TESTO ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TESTO antibody and an TESTO-HRP conjugate. The assay sample and buffer are incubated together with TESTO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TESTO antibody binding site between TESTO from samples and TESTO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TESTO. As more sites are taken up by TESTO from the sample, fewer sites are left that can bind TESTO-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the TESTO concentration in each sample. |
Quality Control on Guinea Pig Testosterone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-103 | |
1:4 | 89-108 | |
1:8 | 86-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TESTO and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Guinea Pig Testosterone ELISA kit
Citations of Guinea Pig Testosterone ELISA kit
E05T0007 has been referenced in the below publications：
Conjugated linoleic acid mitigates testosterone-related changes in body composition in male guinea pigs.
Orchidectomy-induced alterations in volumetric bone density, cortical porosity and strength of femur are attenuated by dietary conjugated linoleic acid in aged guinea pigs.
