E01B0959 Human β parvin ELISA kit
The Human β parvin ELISA kit can be used to identify samples from the Human species. β parvin can also be called PARVB,CGI-56,parvin beta.
Specifications of Human β parvin ELISA kit
Product Information | |
Cat. No. | E01B0959 |
Product Name | Human β parvin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PARVB ELISA kit uses an anti-PARVB antibody and an PARVB-HRP conjugate in a competitive enzyme immunoassay method. PARVB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PARVB antibody binding site between PARVB from samples and PARVB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PARVB. Since the number of sites is limited, as more sites are occupied by PARVB from the sample, fewer sites are left to bind PARVB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PARVB concentration in each sample is interpolated from this standard curve. |
Quality Control on Human β parvin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PARVB and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human β parvin ELISA kit
Citations of Human β parvin ELISA kit
E01B0959 has been referenced in the below publications：
Expression and Significance of Dysferlin and Affixin in Preeclampsia.
