E01B0315 Human β site APP Cleaving Enzyme 1 ELISA kit
The Human β site APP Cleaving Enzyme 1 ELISA kit can be used to identify samples from the human species. β site APP Cleaving Enzyme 1 can also be called BACE1, APP beta secretase, Asp 2, Aspartyl protease 2, BACE, Beta secretase 1, Beta secretase, Beta site amyloid beta A4 precursor protein cleaving enzyme, Beta site amyloid precursor protein cleaving enzyme 1.
Product Information | |
Cat. No. | E01B0315 |
Product Name | Human β site APP Cleaving Enzyme 1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BACE1 ELISA kit uses an anti-BACE1 antibody and an BACE1-HRP conjugate in a competitive enzyme immunoassay method. BACE1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BACE1 antibody binding site between BACE1 from samples and BACE1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BACE1. Since the number of sites is limited, as more sites are occupied by BACE1 from the sample, fewer sites are left to bind BACE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BACE1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BACE1 and analogues was observed. | |
E01B0315 has been referenced in the below publications：
BACE1 levels are increased in plasma of Alzheimer’s disease patients compared with matched cognitively healthy controls.
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