E01A0649 Human 13 hydroxyoctadecadienoic acid ELISA kit
The Human 13 hydroxyoctadecadienoic acid ELISA kit can be used to identify samples from the human species. 13 hydroxyoctadecadienoic acid can also be called 13 HODE, 13(S)-HODE, 13S-HODE.
Specifications of Human 13 hydroxyoctadecadienoic acid ELISA kit
Product Information | |
Cat. No. | E01A0649 |
Product Name | Human 13 hydroxyoctadecadienoic acid ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
13 HODE ELISA kit uses an anti-13 HODE antibody and an 13 HODE-HRP conjugate in a competitive enzyme immunoassay method. 13 HODE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-13 HODE antibody binding site between 13 HODE from samples and 13 HODE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 13 HODE. Since the number of sites is limited, as more sites are occupied by 13 HODE from the sample, fewer sites are left to bind 13 HODE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 13 HODE concentration in each sample is interpolated from this standard curve. |
Quality Control on Human 13 hydroxyoctadecadienoic acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 13 HODE and analogues was observed. | |
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Summary of the Assay Procedure for Human 13 hydroxyoctadecadienoic acid ELISA kit
Citations of Human 13 hydroxyoctadecadienoic acid ELISA kit
E01A0649 has been referenced in the below publications：
Hydrogen-rich water decreases serum low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in patients with potential metabolic syndrome.
