E01H0203 Human 4 Hydroxynonenal ELISA kit
The Human 4 Hydroxynonenal ELISA kit can be used to identify samples from the human species. 4 Hydroxynonenal can also be called 4-Hydroxy-2-Nonenal, 4Hydroxynonenal, 4-Hydroxynonenal, (E)-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA.
Product Information | |
Cat. No. | E01H0203 |
Product Name | Human 4 Hydroxynonenal ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ug/ml |
Sensitivity | 0.1 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 10 ug/mL | 1 vial |
STANDARD E (0.5mL) | 25 ug/mL | 1 vial |
STANDARD F (0.5mL) | 50 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
4 HNE ELISA kit uses an anti-4 HNE antibody and an 4 HNE-HRP conjugate in a competitive enzyme immunoassay method. 4 HNE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-4 HNE antibody binding site between 4 HNE from samples and 4 HNE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 4 HNE. Since the number of sites is limited, as more sites are occupied by 4 HNE from the sample, fewer sites are left to bind 4 HNE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 4 HNE concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 4 HNE and analogues was observed. | |
E01H0203 has been referenced in the below publications：
Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells.
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