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BlueGene Biotech Human 4 Hydroxynonenal ELISA kit (E01H0203)

E01H0203 Human 4 Hydroxynonenal ELISA kit

The Human 4 Hydroxynonenal ELISA kit can be used to identify samples from the human species. 4 Hydroxynonenal can also be called 4-Hydroxy-2-Nonenal, 4Hydroxynonenal, 4-Hydroxynonenal, (E)-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA.

Products

Specifications of Human 4 Hydroxynonenal ELISA kit

Product Information

Cat. No.

E01H0203

Product Name

Human 4 Hydroxynonenal ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ug/ml

Sensitivity

0.1 ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

2.5 ug/mL

1 vial

STANDARD C (0.5mL)

5.0 ug/mL

1 vial

STANDARD D (0.5mL)

10 ug/mL

1 vial

STANDARD E (0.5mL)

25 ug/mL

1 vial

STANDARD F (0.5mL)

50 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

4 HNE ELISA kit uses an anti-4 HNE antibody and an 4 HNE-HRP conjugate in a competitive enzyme immunoassay method. 4 HNE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-4 HNE antibody binding site between 4 HNE from samples and 4 HNE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 4 HNE. Since the number of sites is limited, as more sites are occupied by 4 HNE from the sample, fewer sites are left to bind 4 HNE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 4 HNE concentration in each sample is interpolated from this standard curve.


Quality Control on Human 4 Hydroxynonenal ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 4 HNE and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human 4 Hydroxynonenal ELISA kit

Summary of the Assay Procedure for Human 4 Hydroxynonenal ELISA kit

Citations of Human 4 Hydroxynonenal ELISA kit

E01H0203 has been referenced in the below publications:

Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells.

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