E01E0516 Human 6-kDa Early Secretory Antigenic Target ELISA Kit
The Human 6-kDa Early Secretory Antigenic Target ELISA Kit can be used to identify samples from the human species. 6-kDa Early Secretory Antigenic Target can also be called Early Secreted Antigenic Target 6 kDa.
Product Information | |
Cat. No. | E01E0516 |
Product Name | Human 6-kDa Early Secretory Antigenic Target ELISA Kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| ESAT-6 ELISA kit uses an anti-ESAT-6 antibody and an ESAT-6-HRP conjugate in a competitive enzyme immunoassay method. ESAT-6-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ESAT-6 antibody binding site between ESAT-6 from samples and ESAT-6-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ESAT-6. Since the number of sites is limited, as more sites are occupied by ESAT-6 from the sample, fewer sites are left to bind ESAT-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ESAT-6 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ESAT-6 and analogues was observed. | |
E01E0516 has been referenced in the below publications：
Values of Early Secretory Antigenic Target 6 and Lipoarabinomannan in Diagnosis of Tuberculous Meningitis.
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