E01A0685 Human 6 keto prostaglandin F1a ELISA kit
The Human 6 keto prostaglandin F1a ELISA kit can be used to identify samples from the human species. 6 keto prostaglandin F1a can also be called 6KPGF1a, 6-Keto-prostaglandin F1alpha, 6-Keto-PGF1alpha, 6-Oxo-PGF1alpha, 6-KETOPROSTAGLANDIN F1alpha.
Product Information | |
Cat. No. | E01A0685 |
Product Name | Human 6 keto prostaglandin F1a ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
6KPGF1a ELISA kit uses an anti-6KPGF1a antibody and an 6KPGF1a-HRP conjugate in a competitive enzyme immunoassay method. 6KPGF1a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-6KPGF1a antibody binding site between 6KPGF1a from samples and 6KPGF1a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 6KPGF1a. Since the number of sites is limited, as more sites are occupied by 6KPGF1a from the sample, fewer sites are left to bind 6KPGF1a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 6KPGF1a concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 6KPGF1a and analogues was observed. | |
E01A0685 has been referenced in the below publications：
Correlativity between PGs(6-keto-PGF1α)levels and postpartum hemorrhage caused by uterine inertia.
Experimental study of the intervention effect of Low Molecular Weight Heparin in two routes of administration on rats with acute necrotic pancreatitis.
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