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BlueGene Biotech Human 6 keto prostaglandin F1a ELISA kit (E01A0685)

E01A0685 Human 6 keto prostaglandin F1a ELISA kit

The Human 6 keto prostaglandin F1a ELISA kit can be used to identify samples from the human species. 6 keto prostaglandin F1a can also be called 6KPGF1a, 6-Keto-prostaglandin F1alpha, 6-Keto-PGF1alpha, 6-Oxo-PGF1alpha, 6-KETOPROSTAGLANDIN F1alpha.

Products

Specifications of Human 6 keto prostaglandin F1a ELISA kit

Product Information

Cat. No.

E01A0685

Product Name

Human 6 keto prostaglandin F1a ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

6KPGF1a ELISA kit uses an anti-6KPGF1a antibody and an 6KPGF1a-HRP conjugate in a competitive enzyme immunoassay method. 6KPGF1a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-6KPGF1a antibody binding site between 6KPGF1a from samples and 6KPGF1a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 6KPGF1a. Since the number of sites is limited, as more sites are occupied by 6KPGF1a from the sample, fewer sites are left to bind 6KPGF1a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 6KPGF1a concentration in each sample is interpolated from this standard curve.


Quality Control on Human 6 keto prostaglandin F1a ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 6KPGF1a and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human 6 keto prostaglandin F1a ELISA kit

Summary of the Assay Procedure for Human 6 keto prostaglandin F1a ELISA kit

Citations of Human 6 keto prostaglandin F1a ELISA kit

E01A0685 has been referenced in the below publications:

Correlativity between PGs(6-keto-PGF1α)levels and postpartum hemorrhage caused by uterine inertia.

Experimental study of the intervention effect of Low Molecular Weight Heparin in two routes of administration on rats with acute necrotic pancreatitis.

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