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BlueGene Biotech Human 8 Hydroxy 2 desoxyguanosine ELISA kit (E01H0007)

E01H0007 Human 8 Hydroxy 2 desoxyguanosine ELISA kit

The Human 8 Hydroxy 2 desoxyguanosine ELISA kit can be used to identify samples from the human species. 8 Hydroxy 2 desoxyguanosine can also be called 8 Hydroxyguanosine, 8-Hydroxy-2'-deoxyguanosine, 8-Hydroxydeoxyguanosine, 8 hydroxy 2’ deoxyguanosine, 8 hydroxyguanine, 8 hydroxyguanosine, 8 OHG, 8-OHG, 8OG, 8OHdG, 8OHG, 8 Oxoguanine, 8 hydroxyguanine.

Products

Specifications of Human 8 Hydroxy 2 desoxyguanosine ELISA kit

Product Information

Cat. No.

E01H0007

Product Name

Human 8 Hydroxy 2 desoxyguanosine ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

8 OHdG ELISA kit uses an anti-8 OHdG antibody and an 8 OHdG-HRP conjugate in a competitive enzyme immunoassay method. 8 OHdG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-8 OHdG antibody binding site between 8 OHdG from samples and 8 OHdG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 8 OHdG. Since the number of sites is limited, as more sites are occupied by 8 OHdG from the sample, fewer sites are left to bind 8 OHdG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 8 OHdG concentration in each sample is interpolated from this standard curve.


Quality Control on Human 8 Hydroxy 2 desoxyguanosine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 8 OHdG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human 8 Hydroxy 2 desoxyguanosine ELISA kit

Summary of the Assay Procedure for Human 8 Hydroxy 2 desoxyguanosine ELISA kit

Citations of Human 8 Hydroxy 2 desoxyguanosine ELISA kit

E01H0007 has been referenced in the below publications:

Effect of OGG1 Gene on the Repair Schizandrin B Prevent Benzo [a]-pyrene Induced DNA Oxidative Stress in HTR8-SVneo Cells.

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