E01P0207 Human 8 Isoprostaglandin F2a ELISA kit
The Human 8 Isoprostaglandin F2a ELISA kit can be used to identify samples from the human species. 8 Isoprostaglandin F2a can also be called 8isoPGF2a, 8-iso-PGF2α, 8-Epi-prostaglandin F2α, 8-iso Prostaglandin F2β, (5Z,8β,9α,11α,13E,15S)-9,11,15-Trihydroxyprosta-5,13-dien-1-oic acid1.
Product Information | |
Cat. No. | E01P0207 |
Product Name | Human 8 Isoprostaglandin F2a ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
8isoPGF2a ELISA kit uses an anti-8isoPGF2a antibody and an 8isoPGF2a-HRP conjugate in a competitive enzyme immunoassay method. 8isoPGF2a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-8isoPGF2a antibody binding site between 8isoPGF2a from samples and 8isoPGF2a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 8isoPGF2a. Since the number of sites is limited, as more sites are occupied by 8isoPGF2a from the sample, fewer sites are left to bind 8isoPGF2a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 8isoPGF2a concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 8isoPGF2a and analogues was observed. | |
E01P0207 has been referenced in the below publications：
Hydrogen-rich water decreases serum low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in patients with potential metabolic syndrome.
The evaluation and clinical research of high resolution computed tomography (HRCT) in patients with chronic obstructive pulmonary disease phenotypic.
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