En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Advanced oxidation protein products ELISA kit (E01A0832)

E01A0832 Human Advanced oxidation protein products ELISA kit

The Human Advanced oxidation protein products ELISA kit can be used to identify samples from the Human species. Advanced oxidation protein products can also be called AOPP.

Products

Specifications of Human Advanced oxidation protein products ELISA kit

Product Information

Cat. No.

E01A0832

Product Name

Human Advanced oxidation protein products ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AOPP ELISA kit uses an anti-AOPP antibody and an AOPP-HRP conjugate in a competitive enzyme immunoassay method. AOPP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AOPP antibody binding site between AOPP from samples and AOPP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AOPP. Since the number of sites is limited, as more sites are occupied by AOPP from the sample, fewer sites are left to bind AOPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOPP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Advanced oxidation protein products ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AOPP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Advanced oxidation protein products ELISA kit

Summary of the Assay Procedure for Human Advanced oxidation protein products ELISA kit

Citations of Human Advanced oxidation protein products ELISA kit

E01V0023 has been referenced in the below publications:

Effects of recombinant human growth hormone on the expression of CD47, L-selectin and advanced oxidation protein products in elderly patients with multiple organ dysfunction syndrome.

Research on the relationship between MMP-2, MCP-1, E-seletin, Aopps and p38MAPK signaling pathway in elderly patients with depression.

Comprehensive analysis of oxidative stress indexs in hunman serum with endemic fluorosis of coal burning.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.