Human Alanine Aminotransferase ELISA kit (E01A0388) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Alanine Aminotransferase ELISA kit (E01A0388)

E01A0388 Human Alanine Aminotransferase ELISA kit

The Human Alanine Aminotransferase ELISA kit can be used to identify samples from the human species. Alanine Aminotransferase can also be called Alanine aminotransferase, AAT 1, AAT1, Alanine aminotransferase 1, alanine aminotransferase, ALT/GPT, ALT 1, ALT1, Glutamic alanine transaminase 1, Glutamic pyruvate transaminase, Glutamic pyruvic transaminase 1, Glutamic-alanine transaminase, GPT 1, GPT, GPT1, SGPT, ALAT1.

Products

Specifications of Human Alanine Aminotransferase ELISA kit

Product Information
Cat. No.	E01A0388
Product Name	Human Alanine Aminotransferase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ALT/GPT ELISA kit uses an anti-ALT/GPT antibody and an ALT/GPT-HRP conjugate in a competitive enzyme immunoassay method. ALT/GPT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ALT/GPT antibody binding site between ALT/GPT from samples and ALT/GPT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ALT/GPT. Since the number of sites is limited, as more sites are occupied by ALT/GPT from the sample, fewer sites are left to bind ALT/GPT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALT/GPT concentration in each sample is interpolated from this standard curve.

Quality Control on Human Alanine Aminotransferase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ALT/GPT and analogues was observed.