E01A0777 Human Aldehyde Dehydrogenase ELISA kit
The Human Aldehyde Dehydrogenase ELISA kit can be used to identify samples from the human species. Aldehyde Dehydrogenase can also be called ACDH, ALDH, ALDHA1, CG 3752, CG3752.
Specifications of Human Aldehyde Dehydrogenase ELISA kit
Product Information | |
Cat. No. | E01A0777 |
Product Name | Human Aldehyde Dehydrogenase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 u/L |
Sensitivity | 1.0 u/L |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 u/L | 1 vial |
STANDARD B (0.5mL) | 5.0 u/L | 1 vial |
STANDARD C (0.5mL) | 10 u/L | 1 vial |
STANDARD D (0.5mL) | 25 u/L | 1 vial |
STANDARD E (0.5mL) | 50 u/L | 1 vial |
STANDARD F (0.5mL) | 100 u/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALDH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALDH. Standards or samples are then added to the microtiter plate wells and ALDH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALDH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALDH are added to each well to “sandwich” the ALDH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALDH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Aldehyde Dehydrogenase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ALDH and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Aldehyde Dehydrogenase ELISA kit
Citations of Human Aldehyde Dehydrogenase ELISA kit
E01A0777 has been referenced in the below publications：
High Aldehyde Dehydrogenase Levels Are Detectable in the Serum of Patients with Lung Cancer and May Be Exploited as Screening Biomarkers.
