E01A0774 Human Aldosterone ELISA kit
The Human Aldosterone ELISA kit can be used to identify samples from the human species. Aldosterone can also be called ALD, Aldocorten, Aldocortin, Electrocortin, Reichstein X, 18-Aldocorticosterone, 18-Oxocorticosterone, 11β,21-Dihydroxy-3,20-dioxopregn-4-en-18-al.
Product Information | |
Cat. No. | E01A0774 |
Product Name | Human Aldosterone ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALD ELISA kit uses an anti-ALD antibody and an ALD-HRP conjugate in a competitive enzyme immunoassay method. ALD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ALD antibody binding site between ALD from samples and ALD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ALD. Since the number of sites is limited, as more sites are occupied by ALD from the sample, fewer sites are left to bind ALD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALD concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ALD and analogues was observed. | |
E01A0774 has been referenced in the below publications：
Changes of the Renin-angiotensin-aldosterone Sysetem During the Perioperative Period in Patients with Mitral Valve Replacement its Clinical Significance.
Effect of different hemodialysis modalities on resistin and nutritional status.
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