En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Alpha L fucosidase ELISA kit (E01A0760)

E01A0760 Human Alpha L fucosidase ELISA kit

The Human Alpha L fucosidase ELISA kit can be used to identify samples from the human species. Alpha L fucosidase can also be called AFU, alpha-fucosidase, FUCA.

Products

Specifications of Human Alpha L fucosidase ELISA kit

Product Information

Cat. No.

E01A0760

Product Name

Human Alpha L fucosidase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AFU ELISA kit uses an anti-AFU antibody and an AFU-HRP conjugate in a competitive enzyme immunoassay method. AFU-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AFU antibody binding site between AFU from samples and AFU-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AFU. Since the number of sites is limited, as more sites are occupied by AFU from the sample, fewer sites are left to bind AFU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFU concentration in each sample is interpolated from this standard curve.


Quality Control on Human Alpha L fucosidase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AFU and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Alpha L fucosidase ELISA kit

Summary of the Assay Procedure for Human Alpha L fucosidase ELISA kit

Citations of Human Alpha L fucosidase ELISA kit

E01A0760 has been referenced in the below publications:

The study on the detection of AFP, AFU, CA199 and GPC3 separately or combined in the diagnosis of primary hepatic cancer.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.