E01A0760 Human Alpha L fucosidase ELISA kit
The Human Alpha L fucosidase ELISA kit can be used to identify samples from the human species. Alpha L fucosidase can also be called AFU, alpha-fucosidase, FUCA.
Specifications of Human Alpha L fucosidase ELISA kit
Product Information | |
Cat. No. | E01A0760 |
Product Name | Human Alpha L fucosidase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AFU ELISA kit uses an anti-AFU antibody and an AFU-HRP conjugate in a competitive enzyme immunoassay method. AFU-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AFU antibody binding site between AFU from samples and AFU-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AFU. Since the number of sites is limited, as more sites are occupied by AFU from the sample, fewer sites are left to bind AFU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFU concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Alpha L fucosidase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AFU and analogues was observed. | |
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Summary of the Assay Procedure for Human Alpha L fucosidase ELISA kit
Citations of Human Alpha L fucosidase ELISA kit
E01A0760 has been referenced in the below publications：
The study on the detection of AFP, AFU, CA199 and GPC3 separately or combined in the diagnosis of primary hepatic cancer.
