En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Alpha Synuclein ELISA kit (E01S0033)

E01S0033 Human Alpha Synuclein ELISA kit

The Human Alpha Synuclein ELISA kit can be used to identify samples from the human species. Alpha Synuclein can also be called Alpha SYN, Alpha-synuclein, aSyn, SNCA, synuclein alpha, NACP, PARK1, PARK4, PD1.

Products

Specifications of Human Alpha Synuclein ELISA kit

Product Information

Cat. No.

E01S0033

Product Name

Human Alpha Synuclein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Alpha SYN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Alpha SYN. Standards or samples are then added to the microtiter plate wells and Alpha SYN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Alpha SYN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Alpha SYN are added to each well to “sandwich” the Alpha SYN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Alpha SYN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha SYN concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Alpha Synuclein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Alpha SYN and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Alpha Synuclein ELISA kit

Summary of the Assay Procedure for Human Alpha Synuclein ELISA kit

Citations of Human Alpha Synuclein ELISA kit

E01S0033 has been referenced in the below publications:

May the Evaluation of Nitrosative Stress Through Selective Increase of 3-Nitrotyrosine Proteins Other Than Nitroalbumin and Dominant Tyrosine-125/136 Nitrosylation of Serum a-Synuclein Serve for Diagnosis of Sporadic Parkinson's Disease?

Study of plasma alpha-synuclein oligomers in different Hoehn-Yahr classification Parkinson patients.

Systematic Review on Saliva Biomarkers in Patients Diagnosed with Morbus Alzheimer and Morbus Parkinson.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.