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BlueGene Biotech Human Alpha Synuclein oligomer ELISA kit (E01S0035)

E01S0035 Human Alpha Synuclein oligomer ELISA kit

The Human Alpha Synuclein oligomer ELISA kit can be used to identify samples from the Human species. Alpha Synuclein oligomer can also be called NACP, PARK1, PARK4, PD1.

Products

Specifications of Human Alpha Synuclein oligomer ELISA kit

Product Information

Cat. No.

E01S0035

Product Name

Human Alpha Synuclein oligomer ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Alpha  SYNo ELISA kit uses an anti-Alpha  SYNo antibody and an Alpha  SYNo-HRP conjugate in a competitive enzyme immunoassay method. Alpha  SYNo-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Alpha  SYNo antibody binding site between Alpha  SYNo from samples and Alpha  SYNo-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Alpha  SYNo. Since the number of sites is limited, as more sites are occupied by Alpha  SYNo from the sample, fewer sites are left to bind Alpha  SYNo-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha  SYNo concentration in each sample is interpolated from this standard curve.


Quality Control on Human Alpha Synuclein oligomer ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Alpha  SYNo and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Alpha Synuclein oligomer ELISA kit

Summary of the Assay Procedure for Human Alpha Synuclein oligomer ELISA kit

Citations of Human Alpha Synuclein oligomer ELISA kit

E01S0035 has been referenced in the below publications:

Study of plasma alpha-synuclein oligomers in different Hoehn-Yahr classification Parkinson patients.

A research of blood plasma a-synuclein protein concentration in patient with Parkinson disease.

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