Human Amyloid β Protein 42 ELISA kit (E01A0050) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Amyloid β Protein 42 ELISA kit (E01A0050)

E01A0050 Human Amyloid β Protein 42 ELISA kit

The Human Amyloid β Protein 42 ELISA kit can be used to identify samples from the human species. Amyloid β Protein 42 can also be called Aβ42, beta Amyloid(1-42), beta Amyloid 1-42, beta-Amyloid 1-42, Amyloid 1-42, beta-Amyloid 42, A4, AAA, ABETA, ABPP, AD1, Alzheimers Disease Amyloid Protein, Amyloid B, Amyloid Beta A4 Protein Precursor.

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Specifications of Human Amyloid β Protein 42 ELISA kit

Product Information
Cat. No.	E01A0050
Product Name	Human Amyloid β Protein 42 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Aβ42 ELISA kit uses an anti-Aβ42 antibody and an Aβ42-HRP conjugate in a competitive enzyme immunoassay method. Aβ42-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Aβ42 antibody binding site between Aβ42 from samples and Aβ42-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Aβ42. Since the number of sites is limited, as more sites are occupied by Aβ42 from the sample, fewer sites are left to bind Aβ42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ42 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Amyloid β Protein 42 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Aβ42 and analogues was observed.