E01A1781 Human amyloid beta peptide 25-35 ELISA kit
The Human amyloid beta peptide 25-35 ELISA kit can be used to identify samples from the human species. Amyloid beta peptide 25-35 can also be called Aβ25-35, β-Amyloid peptide(25-35).
Specifications of Human amyloid beta peptide 25-35 ELISA kit
Product Information | |
Cat. No. | E01A1781 |
Product Name | Human amyloid beta peptide 25-35 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Aβ25-35 ELISA kit uses an anti-Aβ25-35 antibody and an Aβ25-35-HRP conjugate in a competitive enzyme immunoassay method. Aβ25-35-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Aβ25-35 antibody binding site between Aβ25-35 from samples and Aβ25-35-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Aβ25-35. Since the number of sites is limited, as more sites are occupied by Aβ25-35 from the sample, fewer sites are left to bind Aβ25-35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ25-35 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human amyloid beta peptide 25-35 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Aβ25-35 and analogues was observed. | |
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Summary of the Assay Procedure for Human amyloid beta peptide 25-35 ELISA kit
Citations of Human amyloid beta peptide 25-35 ELISA kit
E01A1781 has been referenced in the below publications：
The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A.
