Human Angiotensin I Converting Enzyme 2 ELISA kit (E01A0499)

BlueGene Biotech Human Angiotensin I Converting Enzyme 2 ELISA kit (E01A0499)

E01A0499 Human Angiotensin I Converting Enzyme 2 ELISA kit

The Human Angiotensin I Converting Enzyme 2 ELISA kit can be used to identify samples from the human species. Angiotensin I Converting Enzyme 2 can also be called Angiotensin converting enzyme 2, ACE related carboxypeptidase, ACEH, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, angiotensin-converting enzyme 2 precursor, ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2.

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Specifications of Human Angiotensin I Converting Enzyme 2 ELISA kit

Product Information
Cat. No.	E01A0499
Product Name	Human Angiotensin I Converting Enzyme 2 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	250-5000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	250 pg/mL	1 vial
STANDARD C (0.5mL)	500 pg/mL	1 vial
STANDARD D (0.5mL)	1000 pg/mL	1 vial
STANDARD E (0.5mL)	2500 pg/mL	1 vial
STANDARD F (0.5mL)	5000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ACE2 ELISA kit uses an anti-ACE2 antibody and an ACE2-HRP conjugate in a competitive enzyme immunoassay method. ACE2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ACE2 antibody binding site between ACE2 from samples and ACE2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ACE2. Since the number of sites is limited, as more sites are occupied by ACE2 from the sample, fewer sites are left to bind ACE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Angiotensin I Converting Enzyme 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ACE2 and analogues was observed.