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BlueGene Biotech Human Angiotensin I Converting Enzyme 2 ELISA kit (E01A0499)

E01A0499 Human Angiotensin I Converting Enzyme 2 ELISA kit

The Human Angiotensin I Converting Enzyme 2 ELISA kit can be used to identify samples from the human species. Angiotensin I Converting Enzyme 2 can also be called Angiotensin converting enzyme 2, ACE related carboxypeptidase, ACEH, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2,  angiotensin-converting enzyme 2 precursor, ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2.

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Specifications of Human Angiotensin I Converting Enzyme 2 ELISA kit

Product Information

Cat. No.

E01A0499

Product Name

Human Angiotensin I Converting Enzyme 2 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ACE2 ELISA kit uses an anti-ACE2 antibody and an ACE2-HRP conjugate in a competitive enzyme immunoassay method. ACE2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ACE2 antibody binding site between ACE2 from samples and ACE2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ACE2. Since the number of sites is limited, as more sites are occupied by ACE2 from the sample, fewer sites are left to bind ACE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Angiotensin I Converting Enzyme 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ACE2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Angiotensin I Converting Enzyme 2 ELISA kit

Summary of the Assay Procedure for Human Angiotensin I Converting Enzyme 2 ELISA kit

Citations of Human Angiotensin I Converting Enzyme 2 ELISA kit

E01A0499 has been referenced in the below publications:

SERUM LEVELS OF ACE2 AND ANGIOTENSIN-(1-7)IN PATIENTS WITH DIABETIC CARDl0MYOPATHY.

The ACE2 G8790A Polymorphism: Involvement in Type 2 Diabetes Mellitus Combined with Cerebral Stroke.

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