E01A2058 Human anti cyclic citrullinated peptide antibody ELISA Kit
The Human anti cyclic citrullinated peptide antibody ELISA Kit can be used to identify samples from the human species. anti cyclic citrullinated peptide antibody can also be called cyclic citrullinated peptide Ab,ACPA.
Product Information | |
Cat. No. | E01A2058 |
Product Name | Human anti cyclic citrullinated peptide antibody ELISA Kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CCP-Ab ELISA kit uses an anti-CCP-Ab antibody and an CCP-Ab-HRP conjugate in a competitive enzyme immunoassay method. CCP-Ab-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CCP-Ab antibody binding site between CCP-Ab from samples and CCP-Ab-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CCP-Ab. Since the number of sites is limited, as more sites are occupied by CCP-Ab from the sample, fewer sites are left to bind CCP-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCP-Ab concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CCP-Ab and analogues was observed. | |
E01A2058 has been referenced in the below publications：
SERS-based immunoassay of anti-cyclic citrullinated peptide for early diagnosis of rheumatoid arthritis.
Significance of Anti-Carbamylated Protein Antibody Measurement in the Diagnosis of Rheumatoid Arthritis.
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