E01A2036 Human anti lupus anticoagulant antibody ELISA kit
The Human anti lupus anticoagulant antibody ELISA kit can be used to identify samples from the human species. anti lupus anticoagulant antibody can also be called LA IgG, IgG.
Specifications of Human anti lupus anticoagulant antibody ELISA kit
Product Information | |
Cat. No. | E01A2036 |
Product Name | Human Total tumor necrosis factor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LA IgG ELISA kit uses an anti-LA IgG antibody and an LA IgG-HRP conjugate in a competitive enzyme immunoassay method. LA IgG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LA IgG antibody binding site between LA IgG from samples and LA IgG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LA IgG. Since the number of sites is limited, as more sites are occupied by LA IgG from the sample, fewer sites are left to bind LA IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LA IgG concentration in each sample is interpolated from this standard curve. |
Quality Control on Human anti lupus anticoagulant antibody ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LA IgG and analogues was observed. | |
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Summary of the Assay Procedure for Human anti lupus anticoagulant antibody ELISA kit
Citations of Human anti lupus anticoagulant antibody ELISA kit
E01A2036 has been referenced in the below publications：
A Classification of Hashimoto's Thyroiditis Based on Immunohistochemistry for IgG4 and IgG.
