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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Anti-Ovary Antibody, AOAb ELISA kit (E01A2099)

E01A2099 Human Anti-Ovary Antibody, AOAb ELISA kit

The Human Anti-Ovary Antibody, AOAb ELISA kit can be used to identify samples from the human species. Anti-Ovary Antibody, AOAb can also be called AOAb, AOA, oocyte antibody.

Products

Specifications of Human Anti-Ovary Antibody, AOAb ELISA kit

Product Information

Cat. No.

E01A2099

Product Name

Human Anti-Ovary Antibody, AOAb ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AOAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a Ovary antigen and an AOAb-HRP conjugate. The assay sample and buffer are incubated together with AOAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOAb concentration since AOAb from samples and AOAb-HRP conjugate compete for the Ovary antigen binding site. Since the number of sites is limited, as more sites are occupied by AOAb from the sample, fewer sites are left to bind AOAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOAb concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Anti-Ovary Antibody, AOAb ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AOAb and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Anti-Ovary Antibody, AOAb ELISA kit

Summary of the Assay Procedure for Human Anti-Ovary Antibody, AOAb ELISA kit

Citations of Human Anti-Ovary Antibody, AOAb ELISA kit

E01A2099 has been referenced in the below publications:

Anti-Müllerian hormone: its significance in female lupus patients and relation with anti-ovarian antibody.

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