E01A2099 Human Anti-Ovary Antibody, AOAb ELISA kit
The Human Anti-Ovary Antibody, AOAb ELISA kit can be used to identify samples from the human species. Anti-Ovary Antibody, AOAb can also be called AOAb, AOA, oocyte antibody.
Specifications of Human Anti-Ovary Antibody, AOAb ELISA kit
Product Information | |
Cat. No. | E01A2099 |
Product Name | Human Anti-Ovary Antibody, AOAb ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AOAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a Ovary antigen and an AOAb-HRP conjugate. The assay sample and buffer are incubated together with AOAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOAb concentration since AOAb from samples and AOAb-HRP conjugate compete for the Ovary antigen binding site. Since the number of sites is limited, as more sites are occupied by AOAb from the sample, fewer sites are left to bind AOAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOAb concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Anti-Ovary Antibody, AOAb ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AOAb and analogues was observed. | |
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Summary of the Assay Procedure for Human Anti-Ovary Antibody, AOAb ELISA kit
Citations of Human Anti-Ovary Antibody, AOAb ELISA kit
E01A2099 has been referenced in the below publications：
Anti-Müllerian hormone: its significance in female lupus patients and relation with anti-ovarian antibody.
