E01A0074 Human Antistreptolysin O ELISA kit
The Human Antistreptolysin O ELISA kit can be used to identify samples from the human species. Antistreptolysin O can also be called ASO,ASLO.
Specifications of Human Antistreptolysin O ELISA kit
Product Information | |
Cat. No. | E01A0074 |
Product Name | Human Antistreptolysin O ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ASO ELISA kit uses an anti-ASO antibody and an ASO-HRP conjugate in a competitive enzyme immunoassay method. ASO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ASO antibody binding site between ASO from samples and ASO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ASO. Since the number of sites is limited, as more sites are occupied by ASO from the sample, fewer sites are left to bind ASO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASO concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Antistreptolysin O ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ASO and analogues was observed. | |
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Summary of the Assay Procedure for Human Antistreptolysin O ELISA kit
Citations of Human Antistreptolysin O ELISA kit
E01A0074 has been referenced in the below publications：
Clinical significance of anti-streptolysin O and anti-DNA enzyme B in ankylosing spondylitis patients and the association between rheumatoid arthritis with tumor necrosis factor receptor II 196 site polymorphism by meta-analysis.
