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  • bovine serum albumin elisa 1

BlueGene Biotech Human Arginase 1 (Liver type) ELISA kit (E01A0544)

E01A0544 Human Arginase 1 (Liver type) ELISA kit

The Human Arginase 1 (Liver type) ELISA kit can be used to identify samples from the human species. Arginase 1 (Liver type) can also be called liver Arginase, ARG 1, ARG1, ARGI1, Arginase1, Arginase liver, Arginase type I, Arginase I, ArginaseI, Arginase-1, Arginase1, Liver type arginase, Liver-type arginase, Type I arginase.

Products

Specifications of Human Arginase 1 (Liver type) ELISA kit

Product Information

Cat. No.

E01A0544

Product Name

Human Arginase 1 (Liver type) ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Arg1 ELISA kit uses an anti-Arg1 antibody and an Arg1-HRP conjugate in a competitive enzyme immunoassay method. Arg1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Arg1 antibody binding site between Arg1 from samples and Arg1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Arg1. Since the number of sites is limited, as more sites are occupied by Arg1 from the sample, fewer sites are left to bind Arg1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Arg1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Arginase 1 (Liver type) ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Arg1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Arginase 1 (Liver type) ELISA kit

Summary of the Assay Procedure for Human Arginase 1 (Liver type) ELISA kit

Citations of Human Arginase 1 (Liver type) ELISA kit

E01A0544 has been referenced in the below publications:

Macrophages of the Related Factor in the Clinical Value of Tuberculosis .


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